Biomarkers play a crucial role in biomedical research and clinical practice, providing valuable information on disease diagnosis, prognosis, treatment efficacy, and disease progression. Serum and plasma biomarkers, which can be easily accessed and non-invasively obtained, are of particular interest. However, the reliability and accuracy of biomarker measurements can be significantly affected by pre-analytical variables, including sample collection, handling, and storage. Cold storage is a common method employed to preserve serum and plasma biomarker stability, but the optimal storage conditions remain unclear. This literature review aims to critically evaluate the effect of cold storage conditions on the short- and long-term stability of biomarkers in human serum and plasma. Through a systematic review of relevant studies, the review explores the impact of storage temperature, duration, and stabilizing agents on biomarker stability. The findings highlight the variability in stability among different biomarkers and emphasize the need for standardized storage protocols to ensure reliable and accurate biomarker measurements. The review underscores the importance of temperature control, minimizing freeze-thaw cycles, and proper sample handling to maintain biomarker integrity. By providing a comprehensive evaluation of the existing literature, this review identifies gaps and limitations in current knowledge and provides insights into strategies for improving biomarker measurements in clinical practice. The findings have implications for optimizing storage conditions and enhancing the reliability and accuracy of biomarker measurements, ultimately improving patient outcomes.
| Date of Award | 2023 |
|---|
| Original language | English |
|---|
| Awarding Institution | |
|---|
| Supervisor | Berthold Huppertz (Supervisor) |
|---|
The effect of cold storage condition on short-term stability of biomarkers in human serum and plasma
Sanneh, D. (Author). 2023
Student thesis