Overexpression of Eukaryotic Initiation Factor 4F: a Therapeutic Target in Cholangiocarcinoma.

Abstract

Protein synthesis is one of the most critical activities in the living cells. The rate-limiting process in translation is the initiation stage. EIF4F is the key factor in translation initiation regulated by several signaling pathways. In IF staining for eIF4E, eIF4G and 4EBP1, the mean OD in the tumor group had no significant difference compared to the normal group. The p for eIF4E, eIF4G and 4EBP1 at 200× were 0.42, 0.11 and 0.11 respectively, and the p for eIF4E, eIF4G and 4EBP1 at 400× were 0.87, 0.09 and 0.38 respectively. In PLA staining, the eIF4E-eIF4G was significantly upregulated in the CCA cells compared to the normal cholangiocytes, while the eIF4E-4EBP1 decreased dramatically. The p for eIF4E-eIF4G and eIF4E-4EBP1 at 200× were

Date of Award	2019
Original language	English
Awarding Institution	Heidelberg University
Supervisor	Peter Schemmer (Supervisor)