The human high-density lipoprotein (HDL) represents the major cholesterol carrying lipoprotein class in cord blood, while cholesterol is mainly carried by low-density lipoprotein in maternal serum. Changes in maternal lipid metabolism and cholesterol supply might also affect fetal outcome with consequences later in life. Gestational Diabetes Mellitus (GDM), most common complication of pregnancy, is related to postnatal fetal obesity and to higher risk for vascular events. We tested the hypothesis that fetal HDL (fHDL) carries proteins qualitatively and quantitatively different from maternal HDL (mHDL) and that GDM could cause alterations of fHDL proteome and probably its biological function. Shotgun proteomics and biochemical analyses were used to assess composition/function of fHDL and mHDL isolated from uncomplicated and GDM human pregnancies at term of gestation. The pattern of analyzed proteins that were statistically elevated in fHDL (apoE, proteins involved in coagulation, transport processes) suggests a particle characteristic for the light HDL2 sub-fraction. In contrast, proteins that were enriched in mHDL (apoL, apoF, PON1, apoD) have been described almost exclusively in the dense HDL3 fraction and relevant to its anti-oxidative function and role in innate immunity. Strikingly, PON1 mass and activity were 5-fold lower (p
Functionality of fetal high-density lipoprotein particles: Heterogeneity and relationship to Gestation Diabetes Mellitus
Sreckovic, I. (Author). 2014
Student thesis: Doctoral thesis