Prevalence of the PVL-gene in Methicillin-resistant Staphylococcus aureus (MRSA)

Abstract

Infections caused by community-acquired methicillin-resistant Staphylococcus aureus (cMRSA) have emerged worldwide. In the present study a total of 83 non-duplicated MRSA isolates, collected in 2005 at the routine bacteriological laboratory, Institute of Hygiene, Medical University Graz, were investigated for the presence of the PVL-gene by polymerase chain reaction (PCR). Subsequent a fragment analysis was performed using Smal macrorestriction analysis in the pulsed-field gel electrophoresis (PFGE). Among the 83 MRSA isolates 52 (62.6 %) MRSA isolates were from hospitalized patients or patients from clinical ambulatories, 31 (37.4%) MRSA isolates originated from patients treated by local practitioners. Seventeen of the 83 MRSA isolates yielded a positive reaction for the PVL-gene, representing a prevalence rate of cMRSA of 20.5 % among the investigated MRSA. Smal macrorestriction patterns showed a clonal relatedness of cMRSA affecting member of the same family, nosocomial outbreaks of hMRSA could not be detected in any MRSA case in 2005.

Datum der Bewilligung	2007
Originalsprache	Englisch
Gradverleihende Hochschule	Medizinische Universität Graz
Betreuer/-in	Harald Kessler (Betreuer*in) & Robert Krause (Betreuer*in)